Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori

Qingyou Xia[1], Daojun cheng[1], Jun Duan[1], Genhong Wang[1], Tingcai Cheng[1], Xingfu Zha[1], Chun Liu[1], Ping Zhao[1], Fangyin Dai[1], Ze Zhang[1], Ningjia He[1], Liang Zhang[2*], Zhonghuai Xiang[1*]


[1] The Key Sericultural Laboratory of Agricultural Ministry; the Key Laboratory for Sericultural Sciences and Genomics of the Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China.
[2] National Engineering Center for Beijing Biochip Technology, 18 Life Science Parkway, Changping District, Beijing 102206, China
*To whom correspondence should be addressed

Abstract
We designed and constructed a genome-wide microarray with 22,987 70-mer oligonucleotides covering the presently known and predicted genes in the silkworm genome, and surveyed the gene expression in multiple silkworm tissues on day 3 of the fifth instar. Clusters of tissue-prevalent and tissue-specific genes and genes that are differentially expressed in different tissues were identified, and they reflect well major tissue-specific functions on the molecular level. The data presented in this study provide a new resource for annotating the silkworm genome.[Full Text, PDF]